Methanol-induced transcription factor Mpp1 regulates the coordinated expression of multiple genes to achieve a balanced C1 metabolism in the methylotrophic yeast <i>Candida boidinii</i>

Elucidating the molecular mechanism of how methanol-induced gene expression is regulated to attain a balanced one-carbon (C1) metabolism is important for determining the efficiency of heterologous protein production by methylotrophic yeasts. Although transcription factors involved in methanol-induced gene expression in these yeasts have been studied extensively, how these transcription factors regulate the expression of multiple methanol-induced genes in a concentration-regulated methanol induction (CRMI)-dependent manner has been unclear. In this study, we focused on the physiological significance of methanol inducibility of <i>CbMpp1</i> and <i>KpMit1</i> and found that CRMI-dependent <i>CbMPP1</i> and <i>KpMIT1</i> expressions are important for the coordinated regulation of multiple methanol-induced genes to facilitate a balanced C1 metabolism in the methylotrophic yeast.