Identification of essential genes by transposon insertion sequencing and genome-scale metabolic model construction in <i>Streptococcus suis</i>

Anti-microbial resistance (AMR) presents an escalating challenge, making anti-microbial drug development an urgent need. Bacterial essential genes represent promising targets for anti-microbial drugs. However, conventional approaches to identifying bacterial essential genes are time and labor intensive. Techniques such as Tn-seq and GEM construction offer a high-throughput approach for this identification. <i>Streptococcus sui</i>s is an emerging zoonotic bacterial pathogen, posing a big threat to public health as well as the pig industry, and the levels of AMR are increasing. Our study has successfully identified essential genes in <i>S. suis</i>, providing crucial insights for the discovery of new anti-microbial drug targets.