New Polyketides from a Marine Sponge-Derived Fungus, Neopestalotiopsis sp., with Anti-Renal Fibrosis Activity
Sixteen polyketides, including six new compounds (<b>1</b>-<b>2</b>, and <b>5</b>-<b>8</b>), were isolated from the culture of the marine sponge-associated fungus <i>Neopestalotiopsis</i> sp. SCSIO 41422. Their structures were elucidated through NMR, MS spectroscopic analyses, calculated electronic circular dichroism, quantum chemical NMR calculations, and X-ray single-crystal diffraction. To screen and evaluate the inhibitory activity of these polyketides in renal fibrosis, a TGF-<i>β</i>1-stimulated HK-2 cell model was used. All tested compounds (<b>1</b>, <b>5</b>-<b>8</b>, and <b>11</b>-<b>12</b>) at 10 µM showed obvious anti-fibrotic activity by inhibiting TGF-<i>β</i>1-induced <i>α</i>-SMA expression and extracellular matrix production (collagen I and fibronectin). Among them, gamahorin A (<b>1</b>) was shown to be the most potent and the most promising inhibitor against renal fibrosis.
Authors
- Lan Tang (ORCID: https://orcid.org/0000-0002-2345-0886)
- Xiaowei Luo (ORCID: https://orcid.org/0000-0002-2114-1609)
- Jianglian She (ORCID: https://orcid.org/0000-0001-9759-2951)
- Xinlong Li (ORCID: https://orcid.org/0000-0003-1233-2654)
- Xuefeng Zhou (ORCID: https://orcid.org/0000-0001-9601-4869)
- Yonghong Liu (ORCID: https://orcid.org/0000-0001-8327-3108)
- Xinqi Chen (ORCID: https://orcid.org/0000-0003-0413-4893)
- Meiqun Cai
- Rongliang Qiu (ORCID: https://orcid.org/0000-0003-0646-2727)
Institutions
- Guangdong Pharmaceutical University (CN)
- Chinese Academy of Sciences (CN)
- Institute of Oceanology (CN)
- South China Sea Institute Of Oceanology (CN)
- Guangxi University of Chinese Medicine (CN)
- University of Chinese Academy of Sciences (CN)
- Southern Medical University (CN)
Publication Details
- Journal
- Marine Drugs
- Published
- 2025-03-29
- DOI
- https://doi.org/10.3390/md23040148
- Citations
- 1
- Primary Topic
- Fungal Biology and Applications
- Type
- article
- Field-Weighted Citation Impact
- 1.39
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Guangdong Province