Whole genome sequencing of multi-drug resistant Psychrobacter sp. SIT isolated from a sheep hydatid cyst fluid in Mosul city

Background and objectives. Secondary infections of hydatid cysts are most commonly caused by bacteria such as Escherichia coli, Staphylococcus aureus, Pseudomonas species and Salmonella species. To date, no study has reported the isolation of bacterial strains belong to the genus Psychrobacter from hydatid cysts. This study aimed to present the first whole genome sequence of a multidrug-resistant Psychrobacter sp. SIT isolated from a sheep hydatid cyst fluid in Mosul city, Iraq. Materials and methods. The whole-genome of Psychrobacter sp. SIT was sequenced using MiSeq Sequencing System (Illumina, USA). The comprehensive ResFinder 4.0 and CARD tools were used to identify the acquired antibiotic and the quaternary ammonium compounds resistance genes in the genome of Psychrobacter sp. SIT. Results. The sequence genome of Psychrobacter sp. SIT yielded a total of 3,331,920 bp with a DNA GC content of 42.82% and a total of 2,955 protein-coding sequences (CDSs). The results revealed the presence of six genes that corresponded to the predicted resistance phenotypes against five different antibiotic classes, including aminoglycoside (aph(3'')-Ib and aph(6)-Id), aminocyclitol (aadA14), amphenicol (floR), tetracycline (tet(H)), and beta-lactam (blaCARB-16). Moreover, qacG and qacJ genes which confer resistance to benzalkonium chloride (BKC) and cetyltrimethylammonium bromide (CTAB) disinfectants were also reported. The results of isDDH revealed that Pscychrobacter sp. SIT was closely related to Psychrobacter cibarius DSM 16327, with isDDH value of 64.9%. The whole genome sequence of Psychrobacter sp. SIT has been deposited at DDBJ/ENA/GenBank under the accession number JAQGEL000000000. Conclusions. In the current study, an Iraqi bacterium, Psychrobacter sp. SIT, was whole genome sequenced. The ResFinder 4.0 and CARD tools revealed that there were eight antibiotic and quaternary ammonium compounds resistance genes in the genome of Psychrobacter sp. SIT. The isDDH values <70% suggest that Psychrobacter sp. SIT is not only a new strain, but potentially new species of the genus Psychrobacter.