Internal irradiation effect on respiratory activity of tissue fragments of rat small intestine

Objective. To evaluate the respiratory activity of tissue fragments of the small intestine of rats on endogenous and exogenous substrates under conditions of internal irradiation. Materials and methods . The study involved 16 white male rats weighing 180-250 grams. The experimental group of animals received radioactive feed for 30 days, the control group was kept on a standard vivarium diet. The parameters of energy metabolism were studied by polarography on a Record 4. Results . A decrease in tissue respiration indices of the small intestine of rats under conditions of internal irradiation with the incorporation of 137Cs by 32% of the control group was established. Succinic and glutamic acids had a stimulating effect on mitochondrial oxidation of the tissue of intact and experimental animals. Dinitrophenol did not show an uncoupling effect on oxidative phosphorylation in the experimental group. Incoupling was observed under the action of dinitrophenol and the intensity of respiration increased In the control group. Conclusion. The study of the internal irradiation effect on the respiratory activity of tissue fragments of the small intestine of rats in doses actually possible in the population showed high sensitivity of the mitochondrial oxidation system and significant changes in the ratio of oxidized substrates under the influence of incorporated 137Cs. The study showed that the chosen method allows determining the negative impact of internal irradiation on the energy function of the small intestine. Timely detection of disturbances in the energy metabolism of intestinal tissue will optimize the treatment of intestinal pathologies, work out measures to prevent development of destructive processes when using radiotherapy of abdominal organs.