Experimental measurements of glucose concentration in blood with a prototype of optoacustic cytometer, assessment of measurement error

Background and Objectives: Preclinical experimental measurements of blood glucose levels using the optoacoustic method were carried out. The purpose of the work is to record blood glucose levels using the optoacoustic method and obtain a graduated curve. It is necessary to establish the factors influencing the error in measuring blood glucose concentrations. Modern problems arising in the field of optoacoustic studies of blood composition are considered. Materials and Methods: A block diagram of the experimental setup has been developed and a prototype of the device has been created. Methods for collecting and storing blood are described. The process of experimental measurements is given. Experimental studies have been conducted on different age groups of patients with the addition of heparin to stop the clotting process. Results: The obtained profiles of acoustic signals have made it possible to plot the dependence of the amplitude of the acoustic signal in a blood sample on the concentration of glucose in the blood, and the measurement error has been assessed taking into account temperature and concentration factors that influence the result of measuring glucose levels. Conclusion: The prospects for using and comparing the obtained data for in vivo device development have been discussed. The diagnostic accuracy of the optoacoustic method is reduced due to biological variability and heterogeneous tissue composition.