High-resolution chemostratigraphy reveals a large δ13C gradient in the ∼ 1.56 Ga redox-stratified ocean

• Two δ 13 C carb reference curves from fossil-hosting sections are established. • A redox-stratified water column induced an ∼ 7 ‰ δ 13 C org gradient in the ocean. • The sensitivity of shallow water δ 13 C carb was likely buffered by a large DIC pool. • Remineralization of the deep-water DOC pool induced an ∼ 2.5 ‰ δ 13 C DIC gradient. • O 2 concentrations at ∼ 1.56 Ga drove the evolution of early large-scale eukaryotes. Reports of decimeter-scale eukaryotic fossils and oxygenation events in the Gaoyuzhuang Formation (∼1.56 Ga, North China Craton) have provided valuable insight into potential links between life and the environment during the early Mesoproterozoic. However, the detailed nature of this relationship remains unclear, partly due to a limited basin-wide stratigraphic framework. Here, we present high-resolution carbon isotope compositions for carbonate and organic matter (δ 13 C carb and δ 13 C org ) in two fossil-hosting sections, representing shallow and deeper water settings, to calibrate the timing of marine oxygenation and eukaryotic evolution, and to reveal coeval carbon cycle dynamics. Our high-resolution data display a dynamic δ 13 C carb pattern with four perturbations in Gaoyuzhuang members III-IV, and suggest a causal link between oxygenation and eukaryotic evolution during the second perturbation. The δ 13 C carb values exhibit a narrow range, but a distinct ∼ 2.5 ‰ isotopic gradient exists between shallow and deeper water during the third perturbation. By contrast, δ 13 C org values reflect a more stable, but larger, isotopic gradient (∼7‰), implying decoupling of the carbon isotopic system. We propose that the δ 13 C org compositions of shallow and deeper waters were controlled by specific microbial communities in a redox-stratified water column and a larger deep-ocean DOC reservoir, whereas δ 13 C carb sensitivity was buffered by a large DIC reservoir. Our modeling also highlights that the coeval oxygenation events were able to drive the observed short-term δ 13 C carb gradient during the third perturbation. Our findings reveal a direct relationship between environmental change and eukaryotic evolution, with implications for understanding Mesoproterozoic carbon cycle dynamics and paleo-redox conditions.