Antibacterial and Antibiofilm Activity of Some Lactobacillus spp. Isolated from Natural and Commercial Sources Against XDR Pseudomonas aeruginosa Clinical Isolates

Abstract Background: The emergence of biofilm-forming- and antibiotic-resistant Pseudomonas aeruginosa has renewed efforts to identify safe and natural alternative agents such as probiotics. Objectives: This study aimed to assess both the antibacterial and antibiofilm efficacy of some lactobacilli probiotics isolated from natural and commercial sources against pathogenic P. aeruginosa . Materials and Methods: Clinical P. aeruginosa isolates were isolated and identified from Baghdad hospitals, which were later tested for their ability to resist antibiotics and produce biofilms. Lactobacilli species ( Lactobacillus acidophilus and Lactobacillus plantarum ) were also isolated and identified from natural and commercial sources, and their effect on antibacterial and antibiofilm production were studied. Results: The results showed the apparent efficacy of lactobacilli against bacteria and biofilms, and the possibility of using L. acidophilus and L. plantarum as effective probiotics to deal with multidrug-resistant P. aeruginosa , in addition to their role as antibiofilm. The results showed that naturally isolated lactobacilli have more excellent probiotic properties than commercially isolated ones. Finally, the results supported the idea of using probiotics as an alternative to antibiotics to treat antibiotic-resistant pathogenic bacteria, and hence treat some diseases associated with this pathogenic bacterium. Conclusion: The results showed the possibility of using L. acidophilus and L. plantarum as effective probiotics to deal with multidrug-resistant P. aeruginosa , and hence treat some diseases associated with this pathogenic bacterium such as nosocomial infections. Lactobacillus spp. can be good anti-virulence agents (antibiofilm).