The effect of organic solvents on the in vitro transport activity of three OATP isoforms
The uptake activities of OATPs were reduced by methanol and ethanol in a concentration-dependent manner, with IC<sub>20</sub> values of 2.4-3.4 % and 0.51-3.8 % respectively. In contrast, DMSO did not affect the OATP2B1-mediated uptake at the maximum concentration (10 %) of DMSO whereas it exhibited concentration-dependent inhibition for OATP1B1 and 1A2 with IC<sub>20</sub> values of approximately 3 % and 0.7 %, respectively. This study provides useful information regarding experimental conditions for evaluating OATPs activity in vitro.
Authors
- Rie Takahashi (ORCID: https://orcid.org/0000-0003-0255-7040)
- Kazunari Tsujii
- Hisakazu Ohtani (ORCID: https://orcid.org/0000-0002-4089-9407)
- Rina Saito
- Ayuko Imaoka (ORCID: https://orcid.org/0000-0001-9269-0755)
- Takeshi Akiyoshi (ORCID: https://orcid.org/0000-0002-5781-4465)
- Hiroki Kataoka (ORCID: https://orcid.org/0000-0002-8920-5285)
Institutions
- Keio University (JP)
- Keio University Hospital (JP)
Publication Details
- Journal
- Toxicology in Vitro
- Published
- 2025-03-30
- DOI
- https://doi.org/10.1016/j.tiv.2025.106059
- Citations
- 1
- Primary Topic
- Drug Transport and Resistance Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 1.05