DEVELOPMENT OF A METHOD FOR OBTAINING A FLAVONOID COMPLEX FROM THE AERIAL PART OF FERULA SONGARICA PALL. EX SPRENG. WITH ANTIOXIDANT ACTIVITY

The article presents the results of the development of a technique for obtaining phenolic compounds (flavonoids) from the aerial part (stems and leaves) of Ferula songarica Pall. ex Spreng. by maceration method. The quality indicators (loss on drying – 8.15%, total ash – 7.39%, ash insoluble in HCl – 0.29%) of plant materials were established according to the methods of the State Pharmacopoeia of the Republic of Kazakhstan. It was revealed that the extracts contain flavonoids by thin-layer chromatography in appropriate solvent systems using specific reagents. The optimal conditions for the extraction of the amount of flavonoids from the studied plant raw materials were determined: extractant – 70% ethyl alcohol; the ratio of raw materials-extractant – 1:15; extraction time – 24 hours at room temperature. The antioxidant activity of water-ethyl extracts obtained from the aerial part of Ferula songarica Pall. ex Spreng. in the following concentrations of 0.25, 0.5, 0.75 and 1 mg/ml, respectively, was studied in vitro using the Ferric Reducing/Antioxidant Power assay (FRAP). The concentration dependences of the optical density values for plant extracts were obtained in comparison with the standard sample – ascorbic acid (AK). The sequence of increasing antioxidant properties was revealed in the series: 50% ethyl extract > AA > 70% ethyl extract > 90% ethyl extract, while the activity of 50% water-ethyl extract exceeded the standard.