GC MS ANALYSIS, IN SILICO & IN VITRO ANTIDIABETIC STUDIES OF CHITRAKA TRIPHALADI KASHAYA CHOORNA

Chitraka Triphaladi Kashaya Choorna is a traditional Ayurvedic formulation used for various therapeutic purposes, including the management of diabetes mellitus.In this study, we aimed to investigate the chemical composition of Chitraka Triphala Kashaya Choorna and evaluate its potential antidiabetic activity using in silico & in vitro methods.A preliminary phytochemical screening and physico-chemical analysis of ethanolic extracts of choorna was done.Antidiabetic study of Chitraka Triphala Kashaya Choorna was done using alpha amylase assay method.A comprehensive chemical profiling of the Chitraka Triphaladi Kashaya Choorna was performed using gas chromatography -mass spectrometry (GC-MS).In silico studies were conducted to assess the antidiabetic potential of ligands obtained from GC-MS screening.Molecular docking studies were performed using AutoDock Vina.V.1.2.7 against various diabetes target proteins, such as insulin receptor, peroxisome proliferator-activated receptor gamma (PPAR-), dipeptidyl peptidase IV Enzyme were selected.D-allose ligand showed the best binding affinity with the selected proteins.The docking studies aimed to predict the binding affinity and interactions between bioactive compounds present in the formulation and the target protein.These predictions offer valuable insights into the potential mechanisms of action and therapeutic effects of Chitraka Triphaladi Kashaya Choorna in managing diabetes.