Improved breast cancer diagnosis using a CA15-3 capture antibody-lectin sandwich assay
The diagnostic performance of neuCA15-3 was substantially improved by specifically targeting both a CA15-3 protein epitope and a pan-cancer glycan (Neu5Gc) epitope (the specific binding target of SubB2M). The reporter signal generated depends on the colocalization of the cancer antigen protein epitope and the aberrant sialylation of the protein, thus increasing the assay specificity. The presence of multiple Neu5Gc lectin-binding sites per glycoprotein molecule increases signal generation and assay sensitivity. The inclusion of additional cancer biomarkers in a multivariate index assay format may further increase diagnostic performance for breast cancer.
Authors
- Michael P. Jennings (ORCID: https://orcid.org/0000-0002-1027-4684)
- Romina Nabiee (ORCID: https://orcid.org/0000-0001-9806-8481)
- Lucy K. Shewell (ORCID: https://orcid.org/0000-0002-8847-0503)
- Peter T. Simpson (ORCID: https://orcid.org/0000-0002-4816-8289)
- Sara Nikseresht (ORCID: https://orcid.org/0000-0001-7124-6014)
- Megan Moore (ORCID: https://orcid.org/0000-0003-4367-3945)
- Gregory E. Rice (ORCID: https://orcid.org/0000-0001-7177-0246)
- Sunil R. Lakhani (ORCID: https://orcid.org/0000-0003-4067-2760)
- C Day (ORCID: https://orcid.org/0009-0009-4739-2762)
- Ramin Khanabdali (ORCID: https://orcid.org/0000-0003-1420-6934)
- Haarika Chittoory (ORCID: https://orcid.org/0000-0003-2793-3196)
- Amy E. McCart Reed (ORCID: https://orcid.org/0000-0001-5387-2791)
- Leearne Hinch
Institutions
- Griffith University (AU)
- Queensland Health (AU)
- Box Hill Hospital (AU)
- The University of Queensland (AU)
- IQE (United Kingdom) (GB)
- Irvine University (US)
Publication Details
- Journal
- Breast Cancer Research and Treatment
- Published
- 2025-03-27
- DOI
- https://doi.org/10.1007/s10549-025-07672-z
- Citations
- 2
- Primary Topic
- Glycosylation and Glycoproteins Research
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- University of Queensland