Synergistic effects of vitamin C and curcumin in mitigating buprofezin-induced toxicity in non-target animal species

This study aimed to investigate the protective effects of vitamin C and curcumin (CUR) against BPFN-induced toxicity in non-target vertebrate species, focusing on biochemical, hematological, and histological parameters. Laboratory mice were exposed to BPFN at low (50 mg/kg), medium (100 mg/kg), and high (250 mg/kg) doses for 28 days. Three hours post-exposure, the mice received either vitamin C (100 mg/kg), curcumin (100 mg/kg), or a combination of both. Biochemical markers, and diffferent factors in liver and kidney tissues were analyzed. BPFN exposure increased ALT, AST, ALP, total cholesterol, triglycerides, LDH, urea, and creatinine while reducing RBC count, Hb, and HCT. Oxidative stress was confirmed by elevated TBARS and decreased SOD. Combined treatment with vitamin C and CUR significantly mitigated these effects, reducing ALT, and through various activites like SOD. Histological recovery was observed, with tissues appearing similar to controls. The synergistic administration of vitamin C and curcumin effectively countered the toxic effects of BPFN in non-target vertebrates, restoring biochemical, hematological, and histological parameters to near-normal levels. This study highlights the potential of these antioxidants as protective agents against pesticide-induced toxicity. Future research should explore the long-term effects of combined antioxidant treatments on BPFN toxicity.