The Antibacterial and Antioxidant Potential of Prosopis juliflora-Derived CopperNanoparticles against Staphylococcus aureus In Mastitis

Mastitis, an ongoing challenge in the dairy sector causing major economic losses and rising antibiotic resistance.The most common cause of mastitis is Staphylococcus aureus (S. aureus).The major aim of the work is to examine the nature friendly production of copper nanoparticles (CuNPs) using bark extract of Prosopis juliflora (P.juliflora) as a natural reducing and stabilizing agent.The CuNPs were fabricated from an aqueous decoction bark extract of P. juliflora.The characertization of synthsized CuNPs was carried out by UV-Vis spectroscopy, Xray diffraction (XRD), energy dispersive X-ray analysis (EDX), Fourier Transform Infrared Spectroscopy (FTIR), and scanning electron microscopy (SEM).In addition to the color shift from light blue to embraled green, further characterization methods verified that the CuNPs were synthesized with the average size ranged from 10 to 80nm and wide range of morphology.The antibacterial properties of CuNPs were evaluated against S. aureus, demonstrating pronounced zone of inhibition in disc diffusion assay.The maximum zone of inhibition recorded at 600, 400 and 200ppm was 19.50.41,130.82 and 9.10.70mm,respectively.The antioxidant capabilities of the CuNPs were assessed using the DPPH test, demonstrating significant free radical scavenging activity.The UV-Vis spectra of the CuNPs demonstrated a 69.57% reduction in free radical activity, with the highest absorbance occurring at 517nm.This environmentally sensitive synthesis technique provided a sustainable method for nanoparticle production.CuNPs made from P. juliflora can be a good alternative to treat mastitis caused by S. aureus due to their antibacterial and antioxidant potential.