Rational design and pairing of single-domain antibodies for developing rapid detection methods for botulinum neurotoxins A and B
Overall, the TRFICA method we established has a short detection time, is easy to operate, and effectively addresses the challenge of rapid BoNT detection in clinical diagnostics, with significant clinical application value. Furthermore, based on the sequences and binding epitopes of nanobodies, we rationally designed six nanobodies and successfully applied them in the development of various BoNT detection methods. This approach and pipeline provide valuable guidance for the future development of rapid, low-cost detection methods.
Authors
- Xuefang Xu (ORCID: https://orcid.org/0000-0002-3861-8733)
- Lin‐Woo Kang (ORCID: https://orcid.org/0000-0002-1411-3132)
- Wenwen Xin (ORCID: https://orcid.org/0000-0001-5468-2084)
- Xinyu Fang (ORCID: https://orcid.org/0000-0001-6337-8009)
- Guangyu Zhao (ORCID: https://orcid.org/0000-0002-9186-1839)
- Tang Li (ORCID: https://orcid.org/0000-0003-0123-1364)
- Jin Wang (ORCID: https://orcid.org/0000-0002-8810-8862)
- Jing Wang (ORCID: https://orcid.org/0000-0002-3224-3993)
- Wenjing Zhang (ORCID: https://orcid.org/0000-0001-8471-3080)
- Shan Gao (ORCID: https://orcid.org/0000-0003-2083-2616)
- Rui Shi (ORCID: https://orcid.org/0000-0001-6314-2338)
- Jinglin Wang (ORCID: https://orcid.org/0000-0002-1243-8363)
- Ruihua Lv (ORCID: https://orcid.org/0009-0005-6489-5098)
- Jiayan Li (ORCID: https://orcid.org/0009-0000-3436-0483)
Institutions
- Academy of Military Medical Sciences (CN)
- Beijing VDJBio (China) (CN)
- National Institute for Communicable Disease Control and Prevention (CN)
Publication Details
- Journal
- Analytica Chimica Acta
- Published
- 2025-03-29
- DOI
- https://doi.org/10.1016/j.aca.2025.343977
- Citations
- 2
- Primary Topic
- Botulinum Toxin and Related Neurological Disorders
- Type
- article
- Field-Weighted Citation Impact
- 2.78