New rp-hplc method for the simultaneous estimation of dextromethorphan hydrobromide and promethazine in oral dosage forms

Promethazine and dextromethorphan hydrobromide can now be measured simultaneously using a reverse phase high performance liquid chromatography (RP-HPLC) technique that is both sensitive and quick. Using a mobile phase made up of 0.1% O-phosphoric acid and acetonitrile in a 60:40, v/v ratio, chromatographic separation was accomplished on a reverse phase Kinetex C18 column (250 X 4.6 mm, 5 µm). The mobile phase was pumped at a rate of 1.0 milliliters per minute, and a UV detector set to 225 nm was used for detection. The suggested approach was found to be straightforward, quick, accurate, exact, and repeatable. It might be used for routine quality control analysis to determine promethazine and dextromethorphan hydrobromide in pharmaceutical dosage forms at the same time.