Quantitative analysis and antimicrobial efficacy of the ethanol extract obtained from Durio graveolensleaves

Objectives:This study aims to explore the natural antimicrobial properties of ethanol extracts from Durio graveolensleaves (DGL).Methods:DGL underwent preliminary phytochemical screening, along with a quantitative assessment of phenols and flavonoids. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. Antibacterial activity against Gram-positive and Gram-negative bacteria was evaluated using the agar well diffusion method. The microorganisms tested included ATCC strains of Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Streptococcus pyogenes, Neisseria gonorrhoeae, Pseudomonas aeruginosa, and Escherichia coli.Results:DGL demonstrated the highest MIC of 0.1 mg/ml and an MBC of 0.25 mg/ml against the Gram-negative bacteria P. aeruginosaand E. coli. At an MIC of 0.1 mg/ml, DGL produced significant zones of inhibition against P. aeruginosaand E. coliwhen compared to gentamicin.Conclusion:This research indicates that DGL possesses natural antibacterial properties against Gram-negative human pathogens.