National survey of the rat hepatitis E virus in rodents in Spain, 2022 to 2023
BackgroundRat hepatitis E virus (ratHEV) is an emerging virus causing acute and chronic hepatitis in humans. Rats are the main natural reservoir of this pathogen. Large-scale studies assessing ratHEV circulation in rodents in Spain are lacking.AimWe aimed to determine the prevalence of ratHEV in rats in Spain and evaluate potential transmission risk to humans.MethodsWe designed a cross-sectional nationwide study where black (<i>Rattus rattus</i>) and Norway (<i>R. norvegicus</i>) rats were collected and analysed between 2022 and 2023 for ratHEV infection using real-time (RT)-qPCR testing of liver tissue. Sequencing and analysis of ratHEV shedding in faeces were carried out in positive animals.ResultsRatHEV was detected in 125 of the 481 rats analysed, supposing a prevalence of 26.0% (95 CI%: 22.3-30.1). Positive rats were found in urban (25.6%), and farm (29.8%) settings. Black rats (31.3%) had 1.5 times higher odds of being infected by the virus than Norway rats (22.5%) (p = 0.049). Significantly higher prevalence of ratHEV was detected in rodents sampled from southern (31.9%) than northern (17.8%) Spain (p = 0.003). Viral RNA was detected in faeces from 45.5% of infected rats. Phylogenetic analysis evidenced a wide genetic diversity of ratHEV sequences, some showing high homology with ratHEV strains found in patients from Spain.ConclusionsCirculation of ratHEV appears to be heterogeneous and the virus appears to be endemic among rat populations in Spain, highlighting the possible risk of zoonotic transmission of this emerging virus in this country.
Authors
- Víctor Lizana (ORCID: https://orcid.org/0000-0003-2490-6336)
- Moisés Gonzálvez (ORCID: https://orcid.org/0000-0003-0423-3189)
- Marı́a Casares-Jiménez (ORCID: https://orcid.org/0000-0001-5881-4611)
- João R. Mesquita (ORCID: https://orcid.org/0000-0001-8769-8103)
- Antonio Rivero‐Juárez (ORCID: https://orcid.org/0000-0002-5813-6889)
- Adrián Beato‐Benítez (ORCID: https://orcid.org/0000-0001-9608-0022)
- Antonio Rivero (ORCID: https://orcid.org/0000-0002-8643-4250)
- Ignacio García‐Bocanegra (ORCID: https://orcid.org/0000-0003-3388-2604)
- Remigio Martínez (ORCID: https://orcid.org/0000-0002-5908-9447)
- Diana Corona‐Mata (ORCID: https://orcid.org/0000-0002-8059-7171)
- Pilar Foronda (ORCID: https://orcid.org/0000-0001-9526-5113)
- Màrius V. Fuentes (ORCID: https://orcid.org/0000-0002-9508-6390)
- Alba Martí-Marco (ORCID: https://orcid.org/0000-0003-4990-8338)
- Javier Caballero‐Gómez (ORCID: https://orcid.org/0000-0002-6241-3439)
- Francisco Ruiz‐Fons (ORCID: https://orcid.org/0000-0002-0820-5292)
- Pilar Soriano (ORCID: https://orcid.org/0000-0001-6736-1482)
- Lucía Ríos-Muñoz (ORCID: https://orcid.org/0009-0009-0657-1860)
- Dietmar Crailsheim
- Javier Martı́nez (ORCID: https://orcid.org/0000-0002-5912-1128)
- Raúl Cuadrado‐Matías (ORCID: https://orcid.org/0000-0002-4827-6095)
- Tomás Fajardo-Alonso
- Eva Martínez Nevado
Institutions
- Universidad de La Laguna (ES)
- Universitat de València (ES)
- Universidade do Porto (PT)
- Hospital Universitario de Canarias (ES)
- Universidad Cardenal Herrera CEU (ES)
- Instituto de Salud Carlos III (ES)
- Instituto Maimónides de Investigación Biomédica de Córdoba (ES)
- Instituto de Investigación en Recursos Cinegéticos (ES)
- Centro de Investigación Biomédica en Red (ES)
- Hospital Universitario Reina Sofía (ES)
- University of Córdoba (ES)
- Universidad de Murcia (ES)
Publication Details
- Journal
- Eurosurveillance
- Published
- 2025-03-27
- DOI
- https://doi.org/10.2807/1560-7917.es.2025.30.12.2400473
- Citations
- 8
- Primary Topic
- Hepatitis Viruses Studies and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 10.58
Funders
- Universitat de València
- Instituto de Salud Carlos III
- European Regional Development Fund