Effects of Deoxynivalenol Contamination on Growth Performance, Blood Biochemistry, Histology, Metabolomics, and the Microbiota: A Subacute Dose Oral Toxicity Study in Rats

Deoxynivalenol (DON), one of the most common mycotoxins, is frequently found in foods. This study investigated the effects of orally administered DON on the blood biochemical parameters, growth performance, histology, microbial composition, and metabolism of rats. After a 1-week adaptation period, 4-week-old rats were administered 0.9% saline (control), 1 mg/L DON (T1), 10 mg/L DON (T2), or 50 mg/L DON (T3) by gavage for 49 days. The DON-treated groups had significantly lower body weights than the control group (<i>p</i> < 0.05). Blood alkaline phosphatase, phosphate, cholesterol, amylase, and creatinine levels differed significantly between the DON-treated and control groups (<i>p</i> < 0.05). With increasing DON doses, fibrosis and apoptosis were observed in several tissues. In terms of metabolites, the bile acid biosynthesis pathway emerged as a potential biomarker, while the tryptophan metabolism pathway was found to be the most affected. The fecal microbiota showed significant differences in both alpha and beta diversity between the DON-treated and control groups (<i>p</i> < 0.05). In the cecal and fecal microbiota, the relative abundance of Firmicutes increased in the control and T1 groups, whereas Bacteroidota and Campylobacterota were more abundant in the T2 and T3 groups. In conclusion, our results showed that high DON exposure induces several dose-dependent adverse effects on rats.