Methods for Determining Individual Amino Acids in Biological Fluids

Determination of the amino acid composition of biological fluids is of great diagnostic importance. Commonly accepted methods of amino acid analysis include chromatography, electrophoresis and mass-spectrometry. However, for research purposes, to solve specific problems, it is often necessary to determine not the complete amino acid profile, but the concentration of individual amino acids. This review presents literature data analysis on methods used for determining individual amino acids in biological fluids. It is shown that amino acids can be determined by spectrophotometric, electrochemical methods, as well as using a wide range of biosensors, are the detection limit is basically comparable to chromatographic methods of analysis.