Micro-ct Evaluation of the Remineralization Effect of Toothpastes with Different Ingredients on Artificial Initial Enamel Lesions: An ex-vivo study.

Objectives: It was aimed to evaluate the change in mineral density after the application of toothpastes containing fluoride, CPP-ACP, nHAp and CaGP to artificial caries lesions in primary teeth using Micro-CT. Materials and Methods: A total of 40 non-caries primary anterior teeth were randomly divided into 4 groups. A healthy enamel area of 3x1 mm in the center of the 3x3 mm area on the buccal enamel surfaces was used as a control group. The area on the left was covered with nail polish after demineralization. After pH cycling, remineralization materials were applied to the area on the right. Initial enamel lesions were formed on the prepared enamel surfaces using a demineralization solution. The remineralization agents used in the treatment of initial caries lesions were sodium fluoride (NaF), casein phosphopeptide amorphous-calcium phosphate (CPP-ACP), nano-hydroxyapatite (nHAp) and calcium glycerophosphate (CaGP). The changes in mineral density caused by the remineralization agents applied to enamel samples with artificial caries lesions were evaluated by micro-computed tomography (Micro-ct). Data were evaluated with Friedman's Two Way ANOVA test. Results: There was no statistically significant difference between the initial and post-demineralization mineral density values obtained from all groups. There was no statistically significant difference between the remineralization and control groups in terms of mineral density. Conclusions: CPP-ACP, nHAp and CaGP can be used as an alternative to fluoride by supporting remineralization in initial caries lesions of primary teeth.