Anellovirus abundance as an indicator for viral metagenomic classifier utility in plasma samples
Our study underscores the critical impact of classifier selection on diversity indices in metagenomic analyses. Kaiju effectively assigned a wide variety of TTV species, demonstrating it did not require a high volume of reads to capture diversity. Nucleotide-based classifiers like CLARK and Kraken2 showed superior sensitivity, which is valuable for detecting emerging or rare viruses. At the same time, protein-based approaches such as DIAMOND and Kaiju proved robust for identifying known species with low variability.
Authors
- Enéas Carvalho (ORCID: https://orcid.org/0000-0002-8052-0975)
- Maria Carolina Elias (ORCID: https://orcid.org/0000-0002-0501-8280)
- João Paulo Bianchi Ximenez (ORCID: https://orcid.org/0000-0002-6414-5220)
- Luan Gaspar Clemente (ORCID: https://orcid.org/0000-0001-8189-3477)
- Gabriel Montenegro de Campos (ORCID: https://orcid.org/0000-0001-5181-415X)
- Milton Yutaka Nishiyama (ORCID: https://orcid.org/0000-0002-2410-0562)
- Svetoslav Nanev Slavov (ORCID: https://orcid.org/0000-0003-0805-6140)
- Eleonora Cella (ORCID: https://orcid.org/0000-0002-7870-9744)
- Marta Giovanetti (ORCID: https://orcid.org/0000-0002-5849-7326)
- Vagner Fonseca (ORCID: https://orcid.org/0000-0001-5521-6448)
- Alex Ranieri Jerônimo Lima (ORCID: https://orcid.org/0000-0002-5283-7502)
- Sandra Coccuzzo Sampaio (ORCID: https://orcid.org/0000-0001-6257-3531)
Institutions
- University of Central Florida (US)
- Università Campus Bio-Medico (IT)
- Universidade de São Paulo (BR)
- Stellenbosch University (ZA)
- Instituto Butantan (BR)
- Secretaria de Agricultura e Abastecimento (BR)
- Fundação Oswaldo Cruz (BR)
Publication Details
- Journal
- Virology Journal
- Published
- 2025-03-28
- DOI
- https://doi.org/10.1186/s12985-025-02708-8
- Primary Topic
- Animal Virus Infections Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Conselho Nacional de Desenvolvimento Científico e Tecnológico