Importin-9 and a TPR domain protein MpH drive periodic patterning of ciliary arrays in <i>Tetrahymena</i>

We explored how the number of structures is determined in an intracellular organelle series. In Tetrahymena, the oral apparatus contains three diagonal ciliary rows: M1, M2, and M3. During development, the M rows emerge by sequential segmentation of a group of basal bodies, starting with the longest and most anterior M1 and ending with the shortest and most posterior M3. The mpD-1 and mpH-1 alleles increase and decrease the number of M rows, respectively. We identify MpH as a TPR protein and MpD as an importin-9. Both proteins localize to the M rows and form concentration gradients. MpH is a row elongation factor whose loss shortens all M rows and often prevents the formation of M3. MpD limits row initiation after the emergence of M2. MpD could be a part of a negative feedback loop that limits row initiation when M1 assembly is properly advanced. We conclude that the forming oral apparatus has properties of a semi-autonomous intracellular developmental field.

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Publication Details

Journal
The Journal of Cell Biology
Published
2025-03-28
DOI
https://doi.org/10.1083/jcb.202409057
Primary Topic
Protist diversity and phylogeny
Type
article
Field-Weighted Citation Impact
0.00

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article

Importin-9 and a TPR domain protein MpH drive periodic patterning of ciliary arrays in Tetrahymena

Eric S. Cole, Natália E. Bernardes, M. A. B. Hassan, Sunita Sharma et al.
1 links
The Journal of Cell Biology
Protist diversity and phylogeny
article

Importin-9 and a TPR domain protein MpH drive periodic patterning of ciliary arrays in Tetrahymena

Eric S. Cole, Natália E. Bernardes, M. A. B. Hassan, Sunita Sharma, Mireya Parra, Yuh Min Chook, Nathan Michael Chasen, Jacek Gaertig, Anushi Suwaneththiya Deraniyagala, Karl F. Lechtreck, Wolfgang Maier, Deborah Oluwabukola Sowunmi, Elise McCollough Nanista
article en

Abstract

We explored how the number of structures is determined in an intracellular organelle series. In Tetrahymena, the oral apparatus contains three diagonal ciliary rows: M1, M2, and M3. During development, the M rows emerge by sequential segmentation of a group of basal bodies, starting with the longest and most anterior M1 and ending with the shortest and most posterior M3. The mpD-1 and mpH-1 alleles increase and decrease the number of M rows, respectively. We identify MpH as a TPR protein and MpD as an importin-9. Both proteins localize to the M rows and form concentration gradients. MpH is a row elongation factor whose loss shortens all M rows and often prevents the formation of M3. MpD limits row initiation after the emergence of M2. MpD could be a part of a negative feedback loop that limits row initiation when M1 assembly is properly advanced. We conclude that the forming oral apparatus has properties of a semi-autonomous intracellular developmental field.

The Journal of Cell BiologyVol. 224(6)
University of Freiburg (DE), University of Georgia (US), St. Olaf College (US), The University of Texas Southwestern Medical Center (US)
National Science Foundation, Welch Foundation, Bundesministerium für Bildung und Forschung, National Institutes of Health
Openalex Percentile: Top 95%
Protist diversity and phylogeny
DOIOpen Access PDFOpenAlex
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