Low-biomass pyruvate production with engineered Vibrio natriegens is accompanied by parapyruvate formation
We engineered metabolically highly active resting cells of V. natriegens producing pyruvate with high productivity at a low biomass concentration. However, we also found that pyruvate production is accompanied by parapyruvate formation in V. natriegens as well as in a pyruvate producing C. glutamicum strain. Parapyruvate formation seems to be a result of chemical pyruvate conversion and might be supported biochemically by an aldolase reaction.
Authors
- Maurice Hädrich (ORCID: https://orcid.org/0000-0003-2654-6792)
- Jennifer Borger
- Erich Glawischnig (ORCID: https://orcid.org/0000-0001-9280-5065)
- Christoph Gunkel
- Josef Hoff (ORCID: https://orcid.org/0009-0003-4983-1112)
- Bastian Blombach (ORCID: https://orcid.org/0000-0002-2996-2049)
- Felix Thoma (ORCID: https://orcid.org/0000-0002-2457-1057)
- Susanne Müller (ORCID: https://orcid.org/0009-0007-3551-1897)
- Sören-Tobias Vital
- Clarissa Scheuchenegger
Institutions
- Technical University of Munich (DE)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-03-28
- DOI
- https://doi.org/10.1186/s12934-025-02693-1
- Citations
- 3
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 2.42
Funders
- Technische Universität München