DETERMINATION OF THE CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF FRUIT EXTRACTS FROM HIPPOPHAE RHAMNOIDES L. AND CRATAEGUS SANGUINEA L.

Wild-growing berries of Hippophae rhamnoides L. (sea buckthorn) and Crataegus sanguinea L. (hawthorn) were used as plant raw materials for obtaining the extracts. Elemental analysis of the samples was conducted to determine the content of elements such as nitrogen, sulfur, total carbon, and organic carbon. The content of various chemical elements was determined using the inductively coupled plasma mass spectrometry (ICP-MS) method. Liquid extracts were obtained from the fruits of Hippophae rhamnoides (sea buckthorn) and Crataegus sanguinea (hawthorn) using 70% ethanol at a ratio of 1:10. The extracts were characterized by parameters such as the total flavonoid content, quantitative assessment of antioxidant activity. The article describes the results of the quantitative determination of total flavonoids and the assessment of the antioxidant activity of the extracts. The total flavonoid content in the extracts, expressed as quercetin (%), determined by the spectrophotometric method, was as follows: Hippophae rhamnoides L. - 0.37±0.03, Crataegus sanguinea L. - 0.41±0.05. The antioxidant activity of the extracts was studied using the FRAP (Ferric Reducing Antioxidant Power) method, with ascorbic acid serving as the reference antioxidant. As a result of the research, it was found that with an increase in the concentration of the extract from 0.01 to 0.05 mg /ml, the antioxidant activity increases. The antioxidant activity of Crataegus sanguinea L. fruits was found to be higher than that of Hippophae rhamnoides L. fruits. These studies expand the knowledge about the investigated species and can be applied, among other things, to the development of cosmeceutical products with antioxidant activity based on them.Wild-growing berries of Hippophae rhamnoides L. (sea buckthorn) and Crataegus sanguinea L. (hawthorn) were used as plant raw materials for obtaining the extracts. Elemental analysis of the samples was conducted to determine the content of elements such as nitrogen, sulfur, total carbon, and organic carbon. The content of various chemical elements was determined using the inductively coupled plasma mass spectrometry (ICP-MS) method. Liquid extracts were obtained from the fruits of Hippophae rhamnoides (sea buckthorn) and Crataegus sanguinea (hawthorn) using 70% ethanol at a ratio of 1:10. The extracts were characterized by parameters such as the total flavonoid content, quantitative assessment of antioxidant activity. The article describes the results of the quantitative determination of total flavonoids and the assessment of the antioxidant activity of the extracts. The total flavonoid content in the extracts, expressed as quercetin (%), determined by the spectrophotometric method, was as follows: Hippophae rhamnoides L. - 0.37±0.03, Crataegus sanguinea L. - 0.41±0.05. The antioxidant activity of the extracts was studied using the FRAP (Ferric Reducing Antioxidant Power) method, with ascorbic acid serving as the reference antioxidant. As a result of the research, it was found that with an increase in the concentration of the extract from 0.01 to 0.05 mg /ml, the antioxidant activity increases. The antioxidant activity of Crataegus sanguinea L. fruits was found to be higher than that of Hippophae rhamnoides L. fruits. These studies expand the knowledge about the investigated species and can be applied, among other things, to the development of cosmeceutical products with antioxidant activity based on them.