Difenoconazole Degradation by Novel Microbial Consortium TA01: Metabolic Pathway and Microbial Community Analysis

Difenoconazole, a broad-spectrum systemic fungicide, can effectively prevent and control plant diseases such as rice blast, leaf spot, and black spot caused by <i>Colletotrichum godetiae</i>, <i>Alternaria alternata</i>, and <i>Neopestalotiopsis rosae</i>. However, its residual accumulation in the environment may pose potential toxicity risks to non-target organisms. In this study, a highly efficient DIF-degrading microbial consortium TA01 was enriched from long-term pesticide-contaminated soil by a laboratory-based adaptive evolution strategy. The microbial consortium TA01 was able to degrade 83.87% of 50 mg/L of DIF within 3 days. In addition, three intermediate metabolites were identified using HPLC-MS/MS, and the results indicated that the degradation of DIF by microbial consortium TA01 may involve catalytic reactions such as hydrolysis, dehalogenation, and hydroxylation. High-throughput sequencing results showed that <i>Pantoea</i>, <i>Serratia</i>, <i>Ochrobactrum</i>, and <i>Bacillus</i> were the dominant microbial members involved in the degradation process. Finally, bioremediation capacity experiments showed that inoculation with microbial consortium TA01 was able to accelerate the degradation of DIF in the water-sediment system. The findings of this study not only enrich the microbial resources available for DIF degradation but also offer new potential strategies for in situ remediation of DIF contamination.