VEGF-A and TGF-β1 Release in Bovine Bone Scaffolds via Secretome Application

Freeze-Dried Bovine Bone (FDBB) and Decellularized- Freeze Dried Bovine Bone (dc-FDBB) have level low growth factor. Culture of Mesenchymal Stem Cells (MSCs) can used as cell free therapy in form secretome Then combined in biomaterial scaffold for support development engineering network. VEGF-A and TGF-β1 are growth factors that are important in the angiogenesis process in regeneration bones. Compare TGF-β1 and VEGF-A release levels in DBBM, DBBM secretome, FDBB secretome and dc-FDBB secretome. VEGF-A and TGF-β1 release done quantification with ELISA on DBBM, DBBM secretome, FDBB secretome and dc-FDBB secretome. Statistical test Data normality tests (Shapiro-Wilk), homogeneity (Levene), then comparison tests were carried out with one-way Anova and post hoc/ tukey HSD, anova same subject and paired t test. The highest levels of VEGF-A and TGF-β1 release found in FDBB secretome compared to with DBBM, DBBM secretome, and dc-FDBB secretome. There is no difference VEGF-A and TGF-β1 release levels in a way significant between FDBB secretome with dc-FDBB secretome. There is difference VEGF-A and TGF-β1 release levels in a way significant between FDBB secretome with DBBM and DBBM secretome.