Single cell RNA sequencing shows that cells expressing Sox9 postnatally populate most skeletal lineages in mouse bone
In growing bones of mice, multiple cell types contribute to the osteoblast lineage, including growth plate chondrocytes, perichondrial cells and CXCL12-abundant reticular marrow stromal cells. Here we use single-cell RNA sequencing and lineage tracing to show that all these osteoblast precursors, even postnatally, derives from Sox9-expressing progenitors. We also characterize a distinct group of chondrocytes located between the perichondrium and the columns of growth plate chondrocytes that contribute to the osteoblast lineage.
Authors
- Neal Patrick Smith (ORCID: https://orcid.org/0000-0003-1394-3158)
- Alexandra–Chloé Villani (ORCID: https://orcid.org/0000-0001-7461-0408)
- Guping Mao (ORCID: https://orcid.org/0000-0003-4978-4136)
- Ana Clara Morellato Alcantara
- Henry M. Kronenberg (ORCID: https://orcid.org/0000-0001-6905-657X)
- Christopher Janton
- M. A. George
- Kasidet Mankongtreecheep
Institutions
- West Virginia University (US)
- Harvard University (US)
- Sun Yat-sen University (CN)
- West Virginia University Hospitals (US)
- Massachusetts General Hospital (US)
- New York Institute of Technology (US)
- Dana-Farber Cancer Institute (US)
- The First Affiliated Hospital, Sun Yat-sen University (CN)
- Dana-Farber/Harvard Cancer Center (US)
Publication Details
- Journal
- Journal of Bone and Mineral Research
- Published
- 2025-03-26
- DOI
- https://doi.org/10.1093/jbmr/zjaf043
- Citations
- 4
- Primary Topic
- Cancer-related molecular mechanisms research
- Type
- article
- Field-Weighted Citation Impact
- 3.75