Influence of hemolysis, lipemia and bilirubin on biobank sample quality– origin and interference in the use for extracellular vesicle (EV) and MiRNA analyses
The optical absorption spectra measurement is an easy tool for a robust pre-analytic sample controlling for the presence of interferences. Nutrition and anaesthesia were found to be related with lipemia in samples. Hemolysis and lipemia interfered with EV/EV-miRNA analysis. Therefore, the optical absorption spectra pre-analyses should be incorporated in the EV-biobank sampling.
Authors
- İngo Marzi (ORCID: https://orcid.org/0000-0003-3677-2910)
- Liudmila Leppik (ORCID: https://orcid.org/0000-0003-0362-4727)
- Inna Schaible
- Jiaoyan Han
- Dirk Henrich (ORCID: https://orcid.org/0000-0002-9728-4032)
- Birte Weber (ORCID: https://orcid.org/0009-0006-3354-0304)
- Wolfgang Welz
Institutions
- Goethe University Frankfurt (DE)
- University Hospital Frankfurt (DE)
Publication Details
- Journal
- European Journal of Trauma and Emergency Surgery
- Published
- 2025-03-26
- DOI
- https://doi.org/10.1007/s00068-025-02822-w
- Citations
- 2
- Primary Topic
- Clinical Laboratory Practices and Quality Control
- Type
- article
- Field-Weighted Citation Impact
- 2.33