Magnetic beads‐based ligand fishing for rapid screening of dietary α‐glucosidase inhibitory ligands from the rhizome of <scp><i>Alpinia officinarum</i></scp> Hance combined with molecular docking
Aldehyde-activated magnetic beads with 21-carbon linker length and AG<sub>NTC-21</sub>MB are superior to AG<sub>NTC-3</sub>MB in terms of immobilized efficiency, enzymatic activity, and stability. AG<sub>NTC-21</sub>MB-based ligand fishing combined with high-performance liquid chromatography-quadrupole time-of-flight-mass spectrometry and molecular docking constitute an effective and robust bioanalytical technology platform for analyzing antidiabetic composition in A. officinarum. Flavonoids and diarylheptanoids are responsible for the α-glucosidase inhibitory effect of the methanolic A. officinarum extract. The pharmacological and chemical profiles support further research with the aim of developing A. officinarum into functional food for prevention and management of type 2 diabetes and related complications. © 2025 Society of Chemical Industry.
Authors
- Haijie Li (ORCID: https://orcid.org/0000-0003-4908-4615)
- Jian‐Kun Yan
- Wenxiang Hu (ORCID: https://orcid.org/0000-0002-6754-5625)
- Yanshu Zhang (ORCID: https://orcid.org/0000-0003-1207-7251)
- Xi‐Tong Zhang
- Chu Chu (ORCID: https://orcid.org/0000-0003-2938-7862)
- Bing‐Rui Liu (ORCID: https://orcid.org/0000-0001-9243-4217)
Institutions
- North China University of Science and Technology (CN)
- Hebei University of Chinese Medicine (CN)
- Zhejiang University of Technology (CN)
Publication Details
- Journal
- Journal of the Science of Food and Agriculture
- Published
- 2025-03-27
- DOI
- https://doi.org/10.1002/jsfa.14223
- Citations
- 1
- Primary Topic
- Ginger and Zingiberaceae research
- Type
- article
- Field-Weighted Citation Impact
- 1.56