Pencegahan Relaps Postorthodntic dengan Pemberian Kopi yang mengandung kafein pada Tikus

Orthodontic relapse can be defined as the tendency for teeth to return to their pre‐treatment position. Coffee is one substance whose effects on relapse have been extensively studied. The aim of this study was to analyze coffee administration containing caffeine on the osteocytes count in rats. This experimental laboratory study enrolled Spraque dawley rats, with brackets applied using Edgewise slot 0.022” system and a nickel-titanium open coil spring fixed between the lower central incisors for 14 days. Based on Federer’s formula, 36 Spraque dawley were randomly divided by simple random sampling into four groups: Group 1 (no retention), Group 2(retention for 3 days), Group 3(retention for 7 days), and Group 4(retention for 2 weeks). The Spraque-Dawley rats in the four experimental groups were given aquadest, 50 mg, and 100 mg/kg coffee intake ad libitum. Orthodontic relapse was assessed by measuring changes the number osteocytes between the lower central incisors (the relapse side). The number of osteocytes was examined using hematoxylin–eosin staining. Data analysis was conducted with the Two-way analysis of variance (ANOVA) and a post hoc Tukey test. Significant differences were observed in the control and treatment groups and the time of observing osteocytes count levels (P < 0.05). Osteocytes counts in the treatment group were significantly lower than those in the control group. coffee administration containing caffeine encourage postorthodontic relapse prevention by increasing osteocytes count in rats.