Efektivitas Pasta Gigi dengan Kandungan Ekstrak Biji Kopi Robusta (Coffea canephora) dalam Memodulasi Adhesi Streptococcus mutans Terhadap Monosit

S. mutans can invade blood vessels which will trigger a monocyte response with adhesion. Excessive adhesion of S. mutans to monocytes can cause prolonged inflammation and tissue damage so adhesion must be modulated. - The purpose of this study was to examine the ability of toothpaste with robusta coffee bean extract concentrations of 0.78%; 1.56%; 3.12%; and 6.25% in modulating - the adhesion of S. mutans to monocytes. This - is an in vitro laboratory experiment using the post-test only control group design. The sample was divided into 2 groups: Control group (placebo paste) and the treated group (toothpaste containing 0.78%; 1.56%; 3.12%; and 6.25% of robusta coffee bean extract). Placebo paste and toothpaste with robusta coffee bean extract were diluted using RPMI (1:10) before being applied to each sample. Monocyte isolate was incubated with placebo paste and toothpaste with robusta coffee bean extract for 2.5 hours, then - exposured to S. mutans for 4 hours. The adhesion index -was determined by calculating the average number of S. mutans attached to 100 monocytes. The results P<0,05 showed that there were significant differences between the treatment groups. The highest adhesion index was found at a concentration of 0.78% with 5,55% and the lowest at a concentration of 6.25% showed 1,37%. The conclusion of this study based on is that toothpaste with robusta coffee bean extract concentrations of 0.78%; 1.56%; 3.12%; and 6.25% were able to modulate by inhibiting the adhesion of S. mutans to monocytes with the most effective concentration is 6.25%.