Comparing 0.9% sterile saline to phosphate buffered saline as a transport media for Tritrichomonas foetus RT-qPCR testing
The limit of detection for T. foetus RT-qPCR is 1 organism/extraction in phosphate buffered saline (PBS) without prior culture. The objective of this study was to determine if 0.9% sterile saline (SAL), a readily available media, was not inferior to PBS as a transport media for T. foetus RT-qPCR testing at that concentration.
Authors
- E. Heath King
- Magne Thoresen (ORCID: https://orcid.org/0000-0003-1511-5938)
- Donald R. Smith (ORCID: https://orcid.org/0000-0002-4919-1042)
- Duan S. Loy
- T.M. Jumper
Institutions
- University of Nebraska–Lincoln (US)
- Mississippi State University (US)
Publication Details
- Journal
- American Association of Bovine Practitioners Conference Proceedings
- Published
- 2025-03-26
- DOI
- https://doi.org/10.21423/aabppro20249150
- Primary Topic
- Reproductive tract infections research
- Type
- article
- Field-Weighted Citation Impact
- 0.00