A Rapid Method for In Vitro Plant Regeneration of Chia Salvia hispanica L.

Chia Salvia hispanica L. is an annual herbaceous plant. The seeds were shown to have significant macronutrient and micronutrient components. Most of these common macronutrients are proteins, lipids with an appropriate fatty acid composition, ash, carbs, and dietary fiber. This study aimed to find a simple and fast protocol for the regeneration of this plant. The hypocotyls, stem, cotyledons, and young leaves explants of seedlings were grown on Murashige and Skoog (MS) medium fortified with various concentrations of (NAA) and 6-benzyl adenine (BA). Moreover, 2, 4-diclorophenoxyacetic acid (2, 4-D) and kinetin (Kin) were used. Callus was successfully induced from all explants. The most interesting result was the regeneration of shoots from young leaves in one-step during the callus formation. The appropriate medium for shooting was MS enriched with 0.5mg L-1 NAA and 1.0 mg L-1 BA as the percentage of shoot regeneration was (112.5 %). The regenerated shoots were rooted successfully in five days following transferring to agar solidified MS medium. These results revealed the effectiveness of the protocol used in the current study, which is considered the first that was able to produce the important medicinal plant S. hispanica in one-step during callus induction in a short period