Synthesis and characterization of new Thiocarbohydrazone reagent for flow injection spectrophotometric measurement of Co (II) ion an analytical sample

A sample (analyte) is injected into a continuous flow of a carrier solution, where it mixes with chemicals before reaching a detector. This is done automatically using Flow Injection Analysis (FIA). A brand-new thiocarbohydrazone was made by mixing one amount of Methyl thiophen-Thiocarbohydrazone with two amounts of 5-methylthiophen-2-carboxaldehyde in ethanol, We used 1H NMR, 13C NMR and mass spectrometry to figure out the structure of the chemical we made. The FIA method was used to find Cobalt (II) ions in different samples by using this chemical as a spectroscopic reagent, Accurately finding cobalt is very important for environmental and health studies because it is an important element used in many biological and industrial processes.