A Hybrid Plasmonic Nanoplatform for One-Pot DNA and Antibody Detection of Hepatitis B, Monkeypox, and Associated Infections

Currently, nucleic acid and antibody tests for infectious diseases are conducted independently, often resulting in fragmented diagnostics and delayed clinical decision-making. Given the diversity of analytes involved in infectious diseases, comprehensive detection is essential in clinical practice. Here, we introduce One-potNASH, a novel DNA and antibody hybrid plasmonic nanoplatform that unifies nucleic acid and antibody detection within a single assay. By integrating extraction-free thermal lysis of 1 μL of serum, One-potNASH achieves single-DNA copy sensitivity while enhancing antibody detection sensitivity by more than 2 orders of magnitude compared to that of an enzyme-linked immunosorbent assay. Clinically, One-potNASH for hepatitis B, monkeypox (MPX), and associated infections can detect DNA and multiplexed antibodies (anti-HBc, anti-HCV, and anti-HIV for hepatitis B and anti-A29L, anti-H3L, and anti-HIV for MPX) with 100% sensitivity and specificity. This integrated approach addresses critical clinical needs by streamlining workflows, reducing diagnosis time, and enabling a comprehensive evaluation of infectious diseases, thereby facilitating rapid therapeutic interventions and improved patient outcomes.