Spectrophotometric Determination of Tetracycline via Coupling with Diazotized m-Nitroaniline

Background: Tetracycline hydrochloride (TC-HCl) microgram quantities can be determined using a spectrophotometric technique that has been proposed. Methods: The process involves reacting diazotized m-nitroaniline with Tetracycline hydrochloride (TC-HCl) in an alkaline media. Results: At λmax of 382.5 nm, the produced dye's obeyed Beer's law within the range of (1.0 – 30 μg.mL-1). The sensitivity represented by molar absorptivity reached 1.66× 104 l.mol-1. cm-1 and Sandell sensitivity index of 2.8 × 10-2 µg.cm-2 . Depending on the concentration level, the color reaction had a relative standard deviation of 0.357 to 1.26% and a relative accuracy of -2.8 to 0.45%. It was also quite steady. The technique has been effective in of tetracycline-HCl in pharmaceutical. Conclusions: Thus, this technique has been successfully proven to be effective in determining the concentration of Tetracycline hydrochloride (TC-HCl) compound within its pharmaceutical formulations.