The Impact of Moringa oleifera Leaf Extract on Microbial Load in Bovine Semen at Refrigerated Temperatures

Background: The bacterial load on semen sample be negatively impact the fertility of spermatozoa, there by resulting in total breeding failure. The Moringa olifera leaves extract (MOLE) may be a good substitute for the antibiotic component of traditional bovine semen extenders when refrigerated at refrigerated temperature. It may improve semen quality, reduce microbial load and help maintaining fertilization potential. Methods: Present study was investigate the role of hot water Moringa extract supplementation on microbial load in liquid preserved buffalo semen. Agar well diffusion assay and standard plate count test used to evaluate the quantity of live microorganisms in the semen sample at different concentration of MOLE. The anti-bacterial activity for MOLE with 100 µl, 200 µl and 300 µl concentration as compared to control antibiotic solution with streptomycin, ranges from 44.04% to 69.48%, 62.00% to 84.03% and 32.63% to 79.60% againt E. coli, B. cereus, E. faecalis microbes. Result: Our investigation is investigate the effect of Moringa oleifera leaves extract (MOLE) with different concentrations on semen samples at refrigerated temperatures. The higher concentration of M.O.L.E has showed significantly better anti-microbial effect for semen samples preserved up to 72 hrs. The Moringa extract addition in the tris-EYC extender significantly improved semen quality and showed antimicrobial activity during semen preservation at refrigerated temperature.