Protocol for spatial metabolomics and isotope tracing in the mouse brain
Mass spectrometry imaging enables high-resolution spatial chemical mapping, yet its application for dynamic analysis with tracers poses challenges. Here, we present a protocol for spatial metabolomics and isotope tracing in the mouse brain. We describe steps for tracer administration, tissue collection, and cryosectioning. We then detail procedures for matrix application, ion identification, and data analysis. This protocol delivers high-quality spatial metabolomics data and is well suited for region-specific tracing analysis in the brain.
Authors
- M. Nazim Boutaghou
- Isaac Marin‐Valencia
- Carlos Rodríguez‐Navas (ORCID: https://orcid.org/0000-0002-8499-5513)
- Md Amir Hossen
- Manuel González-Rodríguez (ORCID: https://orcid.org/0000-0002-1227-2391)
- Jake Vaynshteyn (ORCID: https://orcid.org/0009-0005-5596-0871)
- Francine Yanchik-Slade (ORCID: https://orcid.org/0000-0002-3816-9990)
- Shinichi Yamaguchi
- Watit Sontising
Institutions
- Shimadzu (Japan) (JP)
- Icahn School of Medicine at Mount Sinai (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-03-27
- DOI
- https://doi.org/10.1016/j.xpro.2025.103716
- Citations
- 2
- Primary Topic
- Metabolomics and Mass Spectrometry Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- March of Dimes Foundation
- National Institutes of Health
- National Institute of Neurological Disorders and Stroke