Structure of the turnip yellows virus particles
Turnip yellows virus (TuYV) is a plant virus infecting important crops such as oilseed rape. TuYV is phloem-restricted and transmitted by aphids. The capsid contains two subunit types: the major capsid protein (CP) and a minor component (RTP∗) which arises from the C-terminal cleavage of a readthrough product (RTP). RTP∗ contains the CP sequence fused with a structured domain, denoted <sup>N</sup>RTD, which is a key determinant of virus transmission. Though both CP and RTP∗ are involved in virus movement and aphid transmission, how RTP∗ is incorporated into the capsid is poorly understood. We present here the structural characterisation, by immunogold labelling and 3D cryo-EM, of the wild-type TuYV and a mutant whose capsid contains the CP only. We show that incorporation of RTP∗ does not impair the capsid structure, and the <sup>N</sup>RTD does not adopt well-defined positions at the capsid surface. The number of incorporated RTP∗s suggests a random insertion.
Authors
- François Lecorre
- Joséphine Lai‐Kee‐Him (ORCID: https://orcid.org/0000-0001-6303-1224)
- Patrick Bron (ORCID: https://orcid.org/0000-0002-3354-9513)
- Stefano Trapani (ORCID: https://orcid.org/0000-0002-2929-6306)
- Catherine Reinbold (ORCID: https://orcid.org/0000-0002-7853-3646)
- V. Ziegler-Graff
- Aurélie Ancelin
- Sylvaine Boissinot (ORCID: https://orcid.org/0009-0005-6455-0516)
- Véronique Brault
- Chloé Fallet
- François Hoh (ORCID: https://orcid.org/0000-0002-2905-7081)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Institut National de la Recherche Agronomique (MA)
- Inserm (FR)
- Université de Montpellier (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Centre de Biologie Structurale (FR)
- Institut de Biologie Moléculaire des Plantes (FR)
- Colmar Inra Research Centre (FR)
Publication Details
- Journal
- Virology
- Published
- 2025-03-27
- DOI
- https://doi.org/10.1016/j.virol.2025.110514
- Primary Topic
- Plant Virus Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- French Infrastructure for Integrated Structural Biology