In-Vitro Analysis of the Anti-Diabetic Potential of Acetone and n-Hexane Extracts of Delonix regia (Gul Mohar) Leaves

Objective: To assess the anti-diabetic activity of leaves extract of D. regia in acetone and n-hexane through in vitro analysis. Methodology: The preclinical study was conducted in the Department of Pharmacology and Therapeutics at Baqai Medical College/University of Karachi, in November 2022. Freshly harvested D. regia leaves were collected identified, and authenticated by the herbarium of the botany department at Karachi University. The extract was concentrated in rotary vacuum evaporator and kept in a desiccator to maintain their integrity and suitability for further applications. In vitro, anti-hyperglycemic studies were conducted using ?-amylase and ?-glucosidase inhibition assays. Results: D. regia leaves extract revealed distinct inhibitory activity of ?-amylase and ?-glucosidase. Acetone extract (25 mg/ml) showed 7.8% and 2.5% inhibition, respectively; while the n-hexane extract (25 mg/ml) demonstrated lower activities, 1.4% and 2.0%, respectively. Conclusion: The present study concludes that polar solvent (acetone) extract of D. regia exhibits more inhibitory potential for ?-amylase and ?-glucosidase as compares to non-polar solvent emphasizing solvent polarity's role in extracting active compounds.