Elevated accumulation of lutein and zeaxanthin in a novel high-biomass yielding strain Dunaliella sp. ZP-1 obtained through EMS mutagenesis

Results in this study support that EMS mutagenesis is an effective breeding approach for further improvement of Dunaliella sp. ZP-1, which is a high-biomass microalgae exhibiting potential to overcome the bottleneck of low biomass of current commercial Dunaliella strains. The mutant tyd4 had higher contents of both lutein and zeaxanthin, whose yield could be further elevated by red light and melatonin. This study provided new microalgae sources for scientific research and technical reference for the bioproduction of natural carotenoids.