Performance evaluation of an improved RAISING method for clonality analysis of bovine leukemia virus-infected cells: a collaborative study in Japan

Bovine leukemia virus (BLV), a retrovirus that is widespread worldwide, causes enzootic bovine leukosis (EBL), a B-cell leukemia/lymphoma with a poor prognosis that ultimately results in death. In Japan, the number of cattle infected with this virus is increasing, and it is estimated more than 35% of cattle are currently infected. Since no vaccines or treatments against BLV infection are currently available, it is important to establish a method of early diagnosis for EBL to reduce economic losses caused by the disposal of EBL cattle in Japan, where a large number of expensive beef cattle are raised. We previously developed Rapid Amplification of the Integration Site without Interference by Genomic DNA Contamination (RAISING), a cost-effective, rapid, and sensitive method for the clonality analysis of BLV-infected cells. Despite its usefulness for the early diagnosis of EBL, RAISING had drawbacks preventing its practical application. Here, we report the development of an improved method, RAISING ver.2, and its performance. Compared to BLV clonality analysis using the previous method, RAISING ver.2 was found to maintain high accuracy and reproducibility despite its simplification. Moreover, its performance was also validated in a multicenter validation study. Taken together, our results strongly suggest that RAISING ver.2 can be fully utilized in clinical practice. Successful commercialization of a RAISING test kit could overcome the concerns of livestock farmers suffering from EBL, thereby promoting a stable supply of Japanese beef, both domestically and internationally.

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Publication Details

Journal
Journal of Veterinary Medical Science
Published
2025-01-01
DOI
https://doi.org/10.1292/jvms.25-0031
Citations
1
Primary Topic
T-cell and Retrovirus Studies
Type
article
Field-Weighted Citation Impact
0.80

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article

Performance evaluation of an improved RAISING method for clonality analysis of bovine leukemia virus-infected cells: a collaborative study in Japan

Yohei Takeda, Masumichi Saito, Hisashi INOKUMA, Shinji Yamada et al.
1 citations
Journal of Veterinary Medical Science
T-cell and Retrovirus Studies
0.80
article

Performance evaluation of an improved RAISING method for clonality analysis of bovine leukemia virus-infected cells: a collaborative study in Japan

Yohei Takeda, Masumichi Saito, Hisashi INOKUMA, Shinji Yamada, Takahiro Matsudaira, Hazuka Yoshida‐Furihata, M. Shiro, Naomi Nojiri, Satoru Konnai, Mana Esaki, Mari Yamamoto, Tatsufumi Usui, Tomohiro Okagawa, Naoya Maekawa, Hidetoshi Higuchi, Yasunori Suzuki, Haruko Ogawa, Shinji Takaı̈, Yasuo Inoshima, Naganori Nao, Hirohisa Mekata, Masaki MAEZAWA, Makoto Ozawa, Satoshi Gondaira, Kenji Murakami, Kazuhiko Ohashi, Michihito Tagawa, Junko Kohara, Misono Tominaga, Kaori Shimizu
article en
1 citations

Abstract

Bovine leukemia virus (BLV), a retrovirus that is widespread worldwide, causes enzootic bovine leukosis (EBL), a B-cell leukemia/lymphoma with a poor prognosis that ultimately results in death. In Japan, the number of cattle infected with this virus is increasing, and it is estimated more than 35% of cattle are currently infected. Since no vaccines or treatments against BLV infection are currently available, it is important to establish a method of early diagnosis for EBL to reduce economic losses caused by the disposal of EBL cattle in Japan, where a large number of expensive beef cattle are raised. We previously developed Rapid Amplification of the Integration Site without Interference by Genomic DNA Contamination (RAISING), a cost-effective, rapid, and sensitive method for the clonality analysis of BLV-infected cells. Despite its usefulness for the early diagnosis of EBL, RAISING had drawbacks preventing its practical application. Here, we report the development of an improved method, RAISING ver.2, and its performance. Compared to BLV clonality analysis using the previous method, RAISING ver.2 was found to maintain high accuracy and reproducibility despite its simplification. Moreover, its performance was also validated in a multicenter validation study. Taken together, our results strongly suggest that RAISING ver.2 can be fully utilized in clinical practice. Successful commercialization of a RAISING test kit could overcome the concerns of livestock farmers suffering from EBL, thereby promoting a stable supply of Japanese beef, both domestically and internationally.

Journal of Veterinary Medical ScienceVol. 87(5)
Kagoshima University (JP), University of Miyazaki (JP), Okayama University of Science (JP), Health Sciences University of Hokkaido (JP), Iwate University (JP), Hokkaido University (JP), National Institute of Infectious Diseases (JP), Advanced Pharma (US), Département Santé Animale (FR), Institut de Recherche Vaccinale (FR), Hokkaido Research Organization (JP), Office of International Affairs (US), Kai Biotech (South Korea) (KR), Gifu University (JP), Tottori University (JP), Kitasato University (JP), Obihiro University of Agriculture and Veterinary Medicine (JP), The University of Tokyo (JP), Rakuno Gakuen University (JP)
Japan Agency for Medical Research and Development, Hokkaido University, Japan Society for the Promotion of Science, Bio-oriented Technology Research Advancement Institution
Openalex Percentile: Top 30%
T-cell and Retrovirus Studies
0.80
DOIOpen Access PDFOpenAlex
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