Analytical Method Development of Bioactive Compounds in Bacopa monnieri Leaf Extract by RP-HPLC

A novel very rapid, sensitive, reverse phase High performance liquid chromatography (RP-HPLC) technique was developed for the quantitative estimation of bioactive compounds in the medicinal plant Bacopa monnieri. Five bioactive compounds namely Bacopasaponin A, Bacoposide I, Bacopaside II, Bacoside A3, Jujobogenin of Bacopasaponin C was resolved by using a mobile phase of phosphate buffer, acetonitrile in the ratio of 60 : 40 % v/v at a flow rate of 1.5ml/min using UV-visible detector at the wavelength of 205nm for quantification. Efficient separation was achieved for Bacopa monnieri on Hypersil BDS C18 (4.6mm X250 mm, 5.0 micron) Column at 30C.With an isocratic elution the separation was obtained in a total run time of 30 mins. The retention time of active constituents was found to be 16.4,9.3,14,13.5,15.4 minutes respectively. Based on the studies it was concluded that the method development of bio active components from Bacopa monnieri were found to be simple, sensitive and inexpensive method. Hence, the development method can be recommended for routine quality control analysis.