CRISPRi-assisted metabolic engineering of cyanobacteria for photosynthetic hyaluronic acid from CO2
This study engineered cyanobacteria for efficient HA biosynthesis using modular gene expression and CRISPR-interference systems. Optimizing heterologous metabolic pathway was key to achieving high-molecular-weight photosynthetic HA production from CO<sub>2</sub>. The findings provide insights into tunable HA production, with future efforts aimed at scaling up photosynthetic HA production for larger-scale applications.
Authors
- Jigyeong Son
- Han Min Woo (ORCID: https://orcid.org/0000-0002-8797-0477)
- Hyun Jeong Lee (ORCID: https://orcid.org/0000-0002-2312-9048)
Institutions
- Sungkyunkwan University (KR)
Publication Details
- Journal
- Journal of Biological Engineering
- Published
- 2025-03-27
- DOI
- https://doi.org/10.1186/s13036-025-00494-z
- Citations
- 2
- Primary Topic
- Photosynthetic Processes and Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- National Research Foundation of Korea