Microsatellite Markers: Cross Species Amplification in Myristicaceae

one of the mega-biodiversity hotspot Western Ghats of India harbours several important and endemic plant taxa and Myristicaceae is one such example also referred as "nutmeg" family one of the ancient families.ive species of Myristicaceae in Western Ghats .It is F occur , among them and var.are exclusively associated with swamps (India's most Gymnacranthera canarica Myristica fatua magnifica Myristica threatened ecosystem).Other three species namely and are non-swampy but are Myristica malabarica M. dactyloides Knema attenuate endemic to Western Ghats.Most of these species are listed in RET data book.In this study, an attempt was made to identify microsatellite markers through cross species amplification in Myristicaceae.Totally, 21 microsatellite (SSR) primers developed for that areVirola sebifera and were examined against three different genera.The study indicated possibility of cross species amplification and use Myristica malabarica of marker for genetic studies in new species.Microsatellite priming sites are more likely to be conserved and thus successful in closely related species (e.g., within subgenera) compared to those that are distantly related (e.g., between genera).Close to 90 percent of species/primer combinations tested within subgenera were successful, a much higher success rate than between genera and geographically distant species.