Exploration of Antidiabetic and Diuretic Activities of Lumnitzera racemosa and Eclipta alba with Molecular Docking Study

The goal of the current study was to look at the leaves of Lumnitzera racemosa (Family: Combretaceae) and aerial part of Eclipta alba (Family: Asteraceae) for its phytochemical constituents and selected pharmacological activities (diuretic and antidiabetic). Diuretic medications are used to treat hypertension. Diabetes and hypertension are two common disease of geriatric patients and our aim was to explore medicinal plants of Sundarbans to find therapeutic of these diseases. The presence of carbohydrates, glycosides, reducing sugar, tannins, flavonoids, alkaloids, proteins, gum, steroids, saponin, and acidic chemicals was shown by phytochemical study of L. racemosa and E. alba showed the existence of tannin, saponin, flavonoid, gum, alkaloid, steroid, and terpenoid. Both plants extract was fractionated depending on polarity using n-hexane (non-polar), ethyl acetate (medium-polar) and water (polar). In the diuretic activity assay using Swiss Albino mice, none of the fractions of L. racemosa and E. alba showed diuretic activity but n-hexane 500 mg/kg of E. alba exhibited little diuretic activity compared to the standard frusemide. Swiss Albino mice were used to assess the oral glucose tolerance test (OGTT) in order to measure antidiabetic activity. In OGTT, water fraction 500 mg/kg of L. racemosa and E. alba showed blood glucose lowering activity compared to the standard Glibenclamide. We had performed in silico study among reported eight (08) compounds of L. racemosa against 1V4S (human glucokinase) protein. In comparison to classic glibenclamide (-8.5 kcal/mol), myricitrin had a good docking score of -8.4 kcal/mol. Based on these findings, we hypothesized that L. racemosa and E. alba could be a possible source of therapeutic leads for hypertension and hyperglycemia.