Lineage labeling with zebrafish <i>hand2</i> Cre and <scp>CreERT2</scp> recombinase <scp>CRISPR</scp> knock‐ins
hand2 progenitors from the lateral plate mesoderm and ectoderm contribute to numerous lineages in the developing embryo. At later stages, hand2-expressing cells are restricted to a subset of lineages in the larva. The endogenous hand2 Cre and CreERT2 drivers establish critical new tools to investigate hand2 lineages in zebrafish embryogenesis and larval organogenesis.
Authors
- Megan Adams
- Karl J. Clark (ORCID: https://orcid.org/0000-0002-9637-0967)
- Maura McGrail (ORCID: https://orcid.org/0000-0001-9308-6189)
- Saulius Sumanas (ORCID: https://orcid.org/0000-0002-8605-6122)
- Christian Mosimann (ORCID: https://orcid.org/0000-0002-0749-2576)
- Jeffrey J. Essner (ORCID: https://orcid.org/0000-0001-8816-3848)
- Iddo Friedberg (ORCID: https://orcid.org/0000-0002-1789-8000)
- Stephen C. Ekker (ORCID: https://orcid.org/0000-0003-0726-4212)
- Parnal Joshi (ORCID: https://orcid.org/0000-0001-6689-3858)
- Hannah R. Moran (ORCID: https://orcid.org/0000-0001-8056-5762)
- Robert L. Lalonde (ORCID: https://orcid.org/0000-0002-1803-0983)
- Nicole K. Restrepo
- Chunyue Yin (ORCID: https://orcid.org/0000-0002-3784-6363)
- Zhitao Ming
- Fang Liu (ORCID: https://orcid.org/0000-0002-8325-1213)
Institutions
- Cincinnati Children's Hospital Medical Center (US)
- Iowa State University (US)
- University of South Florida (US)
- The University of Texas at Austin (US)
- Texas A&M University (US)
- University of Colorado Denver (US)
Publication Details
- Journal
- Developmental Dynamics
- Published
- 2025-03-26
- DOI
- https://doi.org/10.1002/dvdy.70022
- Citations
- 8
- Primary Topic
- Congenital heart defects research
- Type
- article
- Field-Weighted Citation Impact
- 6.45
Funders
- Children's Hospital Colorado
- Iowa State University
- National Heart, Lung, and Blood Institute
- National Institute of General Medical Sciences
- National Institute of Diabetes and Digestive and Kidney Diseases
- Office of Research Infrastructure Programs, National Institutes of Health