Effects of Salt Level and Foliar Putrescine on Yield and Andrographolide Content of Andrographis paniculata

Salt stress is considered the most environmental factor for limiting yield and metabolite content of many plant species. Putrescine is an organic substance consisting of two amine groups. Putrescine plays an important role in many processes in plant growth and stress responds. This study investigated the effects of foliar application of putrescine (C4H12N2) on yield and andrographolide content of Andrographis paniculata under different salinity levels. The research was designed with completely randomized experimental plan (Factorial in CRD) replicating 5 times with 2 factors in Kamphaeng Saen soil experimental sample. Factor A indicated sanitary level which were at 0, 5, and 10 dS/m, and Factor B indicated putrescine concentration which were at 0, 150, and 300 ppm. Harvest was 120 days after planting. Dry and fresh weight of the shoots, H2O2, and andrographolide content were recorded. The results indicated that salinity level caused a significant decrease in fresh and dry weight of the shoot but there was an increase in H2O2 content. The application of Putrescine foliar significantly increased the weight and decreased H2O2 content compared to no Putrescine application. It was also found that the salinity caused the accumulation of andrographolide in Andrographis paniculata. The cumulative amount of andrographolide was significantly at the highest at 150 ppm of Putrescine at 80.7 and 307.1 mg/g, respectively.