Isolation, phylogenetics, and characterization of a new PDCoV strain that affects cellular gene expression in human cells
Among the upregulated genes, <i>FCGR1A</i>, <i>VSIG1</i>, <i>TNFRSF9</i>, and <i>PLCXD3</i> are associated with immunity, inflammation, and lipid catabolism. Moreover, Kyoto Encyclopedia of Genes and Genomes analysis revealed that the upregulated DEGs were significantly enriched in the MAPK, TNF, and NF-κB signaling pathways and viral protein interactions with cytokines and cytokine receptors. Protein-protein interaction networks showed that the upregulated genes <i>CXCL8</i>, <i>DUSP1</i>, <i>PTGS2</i>, and <i>IL15</i> were associated with inflammation and immunity. In addition, the protein levels of p-IRF3, LC3-II, and ACSL4 increased, suggesting that PDCoV infection in Huh7 cells induces an intrinsic immune response, cellular autophagy, and ferroptosis. Collectively, our findings provide new insights into the characteristics and mechanisms of PDCoV infection.
Authors
- Yibo Xi (ORCID: https://orcid.org/0000-0001-9614-6087)
- Zilong Sun (ORCID: https://orcid.org/0000-0003-4907-7271)
- Sheng Niu (ORCID: https://orcid.org/0000-0002-7090-9346)
- Ting Zhang (ORCID: https://orcid.org/0000-0003-3788-6004)
- Aiping Song (ORCID: https://orcid.org/0000-0001-8981-2213)
- Chenyang Wang
- Xiaozhu Yang
- Wei Li (ORCID: https://orcid.org/0000-0002-4506-5516)
- Xuemei Wang (ORCID: https://orcid.org/0000-0003-0298-9695)
- Tao Song (ORCID: https://orcid.org/0000-0001-7370-4277)
- Haidong Wang (ORCID: https://orcid.org/0000-0003-4409-4339)
- Mengyuan Liu (ORCID: https://orcid.org/0000-0001-9268-1576)
- Ding Zhang (ORCID: https://orcid.org/0000-0002-1876-5090)
- Bo Yang (ORCID: https://orcid.org/0000-0001-8392-5663)
- Hanwei Yin
- Farwa Zainab
Institutions
- Shanxi Agricultural University (CN)
- Shanxi Medical University (CN)
- Hebei Normal University of Science and Technology (CN)
Publication Details
- Journal
- Frontiers in Microbiology
- Published
- 2025-03-26
- DOI
- https://doi.org/10.3389/fmicb.2025.1534907
- Citations
- 1
- Primary Topic
- Animal Virus Infections Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.19